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UNITWING/UNESCO
Chairs Programme
Progress Report
I.
Address
Period of activity: 1999
Title of the UNESCO
Chair: Interdisciplinary Biotechnology
Report established
by:
Vittorio Colizzi M.D., Ph.D., Full Professor of General Pathology, UNESCO
Chair in Biotechnology. ICAERI Centre c/o IRCCS L.Spallanzani via Portuense
292, 00149 Rome, Italy. Tel. +39.06.55170430-409 Fax. +39. 06.55170430
E-mail: unesco@uniroma2.it
Web site: http://www.uniroma2.it/unesco
Target groups:
20 graduate students
50 Postgraduate students
2 academics:
Prof. C. D. Pauza , Institute of Human Virology University of Maryland,
USA
Prof. M. Malkovsky, , Medical School, Department of Medical Microbiology
and Immunology University of Wisconsin, USA
Geographical Coverage:
Italy, Poland, USA
Funding sources:
UNESCO Venice Office 25,000 $
University of Rome "Tor Vergata" 25,000 $
Publications:
M.Piacentini
& V.Colizzi Tissue "transglutaminase: apoptosis versus autoimmunity.
Immunol. Today, (1999); 20:130-133
Kornfeld H.,
Mancino G., and Colizzi V. The role of macrophage cell death in Tuberculosis.
Cell Death Differ, (1999); 6: 71-78.
M.L. Gougeoun,
S.Boulier, V.Colizzi and F.Poccia. NKR-mediated control of gd T cell immunity
to viruses. Microbes and Infection, (1999);1 219-226
Bergamini,
A., Faggioli, E., Bolacchi, F., Gessani, S., Cappannoli, L., Uccella,
I., Demin, F., Capozzi, M., Cicconi, R., Placido, R., Vendetti, S., Mancino,
G., Colizzi, V., Rocchi, G. Enhanced production of TNF-a and Il-6, due
to prolonged cytokine response to LPS stimulation, in cultured human macrophages
infected in vitro with HIV-1. J. Infect. Dis, (1999); 179, 832-42
F. Poccia,
L. Battistini, B. Cipriani, G. Mancino, F. Martini, M.L. Gougeon and V.
Colizzi. Phosphoantigen-reactive Vg9Vd2 T Lymphocytes Suppress in vitro
Human Immunodeficiency Virus Type 1 Replication by Cell and Antiviral
Factors Including CC-Chemochines J. Inf. Dis. (1999); 180:858-61
F. Poccia,
M. Malkovsky, A. Pollak, V. Colizzi, G. Sireci, A. Salerno and F. Dieli.
In vivo gd T cell priming to mycobacterial antigens by primary M.tuberculosis
infection and exposure to nonpeptidic ligands. Molecular Medicine (1999);
5: 471-476
M. Fraziano,
G. Cappelli, M. Santucci, F. Mariani, M. Amicosante, M. Casarini, S. Giosuè,
A Bisetti and V.Colizzi. Expression of CCR5 Is Increased in Human Monocyte-Derived
Macrophages in the Course of in Vivo and in Vitro Mycobacterium tuberculosis
Infection. AIDS Res. Hum. Retrov.(1999); 15, 869-874
G.Antonelli,
E.Riva, F.Maggi, M.L.Vatteroni and E.Simeoni. Influence of Hepatitis C
Virus (HCV) Genotype, HCV RNA Load, and Alanine Aminotransferase Level
on Reduction of HCV RNA after a Single Administration of Interferon a.
J.Inf Dis (1999); 180, 1411-12
S.Uccini, E.Riva,
G.Antonelli, G.D'offizi, A.Prozzo, A.Angelici, A.Faggioni, A.Angeloni,
M.R.Torrisi, M.Gentile, C.D.Baroni and L.P.Ruco. The Benign Cystic Lymphoepithelial
Lesion of the Parotid Glands Is a Viral Reservoir in HIV Type 1-Infected
Patients. AIDS Res. Hum Retrov (1999); 15, 1339-1344
Antonelli and
F.Dianzani. Development of antibodies to interferon beta in patients:
technical and biological aspects. Eur.Cytokine Netw. (1999); 10, 413-422
G.Antonelli,
E. Simeoni, F.Bagnato, C.Pozzilli, O.Turriziani, R.Tesoro, P.Di Marco,
C.Gasperini, C.Fieschi, F.Dianzani. Further study on the specificity and
incidence of neutralising antibodies to interferon (TNF) in relapsing
remitting multiple sclerosis patients treated whit IFN beta -1a or IFN
beta -1b. J.Neurol Sci. (1999); 168, 131-136
Multimedia
Material
Title of activity: International Centre for AIDS and Emerging
& Re-emerging Infections (ICAERI) Annual Report 1998-99
Year: 1999
Type of material: Book and CD ROM (Web Page http://www.uniroma2.it/unesco/centro
Language: English
This CD-ROM describes activities carried out by the (ICAERI) Centre at
the National Institute for Infectious Diseases "L.Spallanzani"
in Rome, including the activity carried out by the Chair in the Interdisciplinary
Biotechnology for the year 1998.
Title of
activity: Biotecnologia
Producer: Scientific Park, University of Rome Tor Vergata
Year: 1999
Type of material: CD ROM
Language: Italian
This CD-ROM illustrates the different fields of Biotechnology (agriculture,
pharmaceutical products, gene therapy, etc.) with particular references
to the teaching aspects of Biotechnology and the possibility of working
activity in the industries near Rome.
II.
Available resources:
1. Human resources
- For the administration of the UNESCO Chair or Network Budget
1999: 25,000 $;
- For the administration of the UNESCO Chair or Network; two visiting
professor: Prof. C.D. Pauza 8,000 $ - Prof. M. Malkovsky 6,000 $. Total
14,000 $;
- For information and documentation activities ( secretary, print
posters and certificate of the Courses) 6,000 $;
- Others: Missions Prof. Colizzi and local speakers 1,000 $ (Unesco);
- Others: Scientific publication on J. Biol. Regul. Homeost. Agent.
(Unesco - IRCCS L.Spallanzani) special issue 4,000 $.
2. Material resources
- For the teaching/training/research activities; slides, photocopies,
transparency film and CD ROM 10,000 $ (University);
- for the laboratory research: culture media, reagents, plastics,
etc. 15,000 $ (University).
III.
Activities
Target groups
- Graduate students 20
Postgraduate students 50
Academics 2
Geographical coverage
National, Italy
Regional, Europe
Funding
sources
|
Type
of Organisation / Institution |
Period |
Amount |
UNESCO
Contribution |
International
Agency |
1999/2000 |
$
25,000 |
University
of Rome "Tor Vergata" |
|
1999 |
$
25,000 |
1. Education/Training/Research:
This Chair as an Interuniversitary activity associated not only to the
University of Rome Tor Vergata but also to the University of Roma TRE
and then to the University of Rome La Sapienza. The objective is to educate
students of the three governmental universities in Rome enrolled in different
areas (Biology , Medicine, Chemistry, Economy, Law) to understand the
complexity of Biotechnology.
2. Conferences/Meetings:
BIOROMA 2000 - Biotechnology: new opportunities for local development
in Europe Rome 30-31 March 2000.
3. Mission/Travels abroad:
Prof. V. Colizzi: Mission to Madison WI USA (22/11/99) for take contacts
with Prof. Malkovsky and Prof.Pauza and for the development of the teaching
program concerning the UNESCO Courses in Biotechnology.
4. Visiting Professor/Fellowships:
Prof. C.D.Pauza.
"Course on scientific Management Stile and Outcome Measure in Biomedical
Research"
Upon decision of the Rector of the University of Rome Tor Vergata, the
Chair was associated during the academic year 1999/2000 with
International Centre for AIDS and Emerging & Re-emerging Infections
(ICAERI) at the IRCCS L.Spallanzani in Rome. (see Annex 1) directed by
Professor Vittorio Colizzi. The ICAERI Centre has been inaugurated on
March the 25th 1998, as result of an agreement between the Institute for
Infectious Diseases "Lazzaro Spallanzani", the UNESCO and the
World Foundation for AIDS Research and Prevention. ICAERI originates from
the CNR-UNESCO agreement to increase the cooperation in scientific research
and science communication in the field of AIDS, considering the worldwide
impact of this infectious disease.
More specifically, the following tasks of the Chair were defined: organisation
of the course on Scientific Management Styles and Outcome Measure in Biomedical
Research. The chairholder has been given to Professor C.D. Pauza, Ph.D,
Institute of Human Virology, University of Maryland, USA. Moreover, Dr.
F.Martini, Dr. M. Mattei, and Dr. F.Poccia from the group of Professor
Colizzi supported the activities of the Chair. The official language was
English, with introduction and summary additionally in Italian language.
The main objectives of the UNESCO Course on Scientific Management included:
Orientation
Introduction to Strategic Planning
S.W.O.T. analysis exercise
Annual Operating plan and performance evaluation
Writing a strategic plan for the Infectious diseases Institute
Technology transfer, conflict of interest, patent and licensing
development
Prof. M.
Malkovsky, M.D., PhD. "Advanced Course on Immunology of Infectious
diseases"
Upon decision of the Rector of the University, the second part of the
Chair was also associated during the academic year 1999/2000 with
International Centre for AIDS and Emerging & Re-emerging Infections
(ICAERI) at the IRCCS L.Spallanzani in Rome. The chairholder was Professor
Prof. M. Malkovsky, M.D., PhD, FRCPath Professor of Immunology, Medical
School University of Wisconsin, USA, Dr. F.Poccia from the group of Professor
Colizzi supported the activities of the Chair. The official language was
English, with introduction and summary additionally in Italian language.
The UNESCO Advanced Course in Immunology of Infectious Diseases was taught
by Prof. Malkovsky and the main objectives of the course included:
1. Discussing the state-of-the-art basic and applied knowledge
of cellular and molecular immunology and pathogenic and protective mechanisms
in various infectious diseases.
2. Learning to apply the new concepts and technology to the diagnosis,
prevention and therapy of infectious diseases.
3. Promoting the view that strong links between basic sciences,
clinical diagnosing and therapies of infectious diseases are essential
for improving public health.
The target audience included (but was not limited to):
1. MDs, PhDs and other postdoctoral scientists, who wished to develop
new career objectives in the field of infectious diseases.
2. PhD students and other predoctoral scientists interested in
immunology.
3. Scientists seeking a better understanding of basic and applied
immunology.
4. Researchers interested in different views on how to conduct
research.
The course was divided into four naturally-overlapping and each-other-complementing
sections:
Section 1 - How
to select research problems
I: Basic cellular and molecular mechanisms
II: Activation versus tolerance
Antigen presentation and memory cells
Activation, anergy, immunological tolerance, ignorance, hypo- and
unresponsiveness
TCRs, accessory and co-stimulatory molecules
Section 2 - How to select research problems
III: Immunopathogenesis
Pathogenic insults due to acute infections.
Pathogenic insults due to chronic infection.
Immunopathogenesis of HIV/SIV infections
Immunopathogenesis of mycobacterial infections
Section 3 - How to write a scientific paper
High impact journals
Other journals
There are no good or bad journals; there are only good and bad papers
Section 4 - How to write an application for a scientific grant
CNR, MURST, ISS
EU and NIH
International agencies (WHO, UNESCO, etc.)
Industrial
The main challenge from the teacher's standpoint was that the 35 participants
had all displayed quite varied levels of the knowledge of immunology,
ranging from 'practically zero' to 'reasonably sophisticated'. This fact
was established by filling a questionnaire before the beginning of the
course and discussing the subject with the participants. The participants
were asked to describe briefly a) their knowledge of immunology and b)
their expectations (what exactly they would like to learn).
Based on the assessment of participants' knowledge, the initial section
of the course included an analysis of the components of the immune system,
principles of innate and adaptive immunity, and the recognition and effector
mechanisms of adaptive immunity. Subsequently, it was discussed the induction,
measurement, and manipulation of the immune response, structures of antibody
molecules and immunoglobulin genes, antigen recognition by T lymphocytes,
and signalling through lymphocyte receptors. Then it was focused (as requested
by some students) on the thymus, the development of T cells in the thymus,
T-cell receptor gene (TCR) rearrangements and TCR expression, positive
and negative selection of T cells, general properties and production of
armed effector T cells, T-cell mediated cytotoxicity, macrophage activation
by armed CD4 TH1 cells, generation of B cells, selection of B cells, B-cell
heterogeneity, antibody production by B lymphocytes, the distribution
and function of immunoglobulin isotypes, Fc receptor-bearing accessory
cells in humoral immunity and the complement system in humoral immunity.
The next discussions were focused on the immune system in health and disease.
Specifically, host defence against infection and failures of host defence
mechanisms (inherited versus acquired) were discussed with many specific
examples (the acquired immune deficiency syndrome caused by HIV was stressed
in particular). Then it was analysed in detail allergy and hypersensitivity,
immune responses in the 'putative' absence of infection (autoimmunity,
transplant rejection and tolerance, and manipulation of the immune response.
This last immunological area of discussions focused on extrinsic regulation
of unwanted immune responses, using the immune response to attack tumours,
and manipulating the immune response to fight infections (vaccines and
related topics).
In addition, it was discussed in a great detail five original articles
(in five different discussion sessions, which followed the relevant lecture):
1. Wells AD, Li XC, Li Y, Walsh MC, Zheng XX, Wu Z, Nunez G, Tang
A, Sayegh M, Hancock WW, Strom TB, Turka LA. Requirement for T-cell apoptosis
in the induction of peripheral transplantation tolerance. Nat Med. 1999
Nov;5(11):1303-1307.
2. Li Y, Li XC, Zheng XX, Wells AD, Turka LA, Strom TB. Blocking
both signal 1 and signal 2 of T-cell activation prevents apoptosis of
alloreactive T cells and induction of peripheral allograft tolerance.
Nat Med. 1999 Nov;5(11):1298-1302.
3. Tamada K, Shimozaki K, Chapoval AI, Zhu G, Sica G, Flies D,
Boone T, Hsu H, Fu YX, Nagata S, Ni J, Chen L. Modulation of T-cell-mediated
immunity in tumour and graft-versus-host disease models through the LIGHT
co-stimulatory pathway. Nat Med. 2000 Mar;6(3):283-289.
4. Chen CH, Wang TL, Hung CF, Yang Y, Young RA, Pardoll DM, Wu
TC. Enhancement of DNA vaccine potency by linkage of antigen gene to an
HSP70 gene. Cancer Res. 2000 Feb 15;60(4):1035-1042.
5. Randolph DA, Huang G, Carruthers CJ, Bromley LE, Chaplin DD.
The role of CCR7 in TH1 and TH2 cell localization and delivery of B cell
help in vivo. Science. 1999 Dec 10;286(5447):2159-2162.
First, one of the students presented a detailed account of the given article
(background, significance, data analysis, conclusions) and then we designed
strategies (and discussed their feasibility) for continuing this line
of research. This is one of the best way of learning "how to select
a scientific problem", which is an important part of any successful
scientific process, but is usually severely neglected by regular university
courses. Similarly, four invited speakers (Drs. M.-T. De Magistris, C.
Saltini, C.D. Pauza and F. Poccia) presented their original research data,
and subsequently we discussed their research and its possible continuation
pathways exactly as we did with the recent original research papers.
Finally, the course included two sessions on "How to write a scientific
paper" (original research papers versus reviews versus textbook articles)
and "How to write an application for a scientific grant". It
was focused our discussions on particular requirements for each entity
as well as on common pitfalls during the writing process such as incomprehensibility,
overinterpretation of data, discordance of data and conclusions, poor
quality (figures, photos, drawings), missing legends, insufficient details,
important data not shown, mixing 'Introduction', 'Results' and 'Discussion'
sentences with numerous examples.
5. Information
and documentation activities: Organising secretary of the UNESCO Courses
by Dr. C. Costa (ICAERI) IRCCS L.Spallanzani.
IV.
Impact:
Biotechnology is one
of the most relevant technologies in the next millennium. Most of the
basic knowledge in Biology and Medicine can be exploited in term of utilisation
and application. Due to the complexity of the society and to the different
sensibility in term of ethic and philosophy an integrated approach to
Biotechnology is required. These UNESCO Courses in Biotechnology represented
a concrete answer to the limit of single disciplines and monotematic teaching
course in a complex aspect of modern life such as Biotechnology.
The activation of the UNESCO Interdisciplinary Chair in Biotechnology
at the University of Rome " Tor Vergata ", in the context of
the Project UNESCO UNI/TWIN, has represented one occasion to see different
methodologies of teaching in a complex field such as biotechnology. Theoretical
and practical aspects of Biotechnology have been analysed in the context
of modern knowledge and their industrial applications.
Two UNESCO Courses in Biotechnology were carried out at the University
of Rome " Tor Vergata, one course has been dedicated to the Immunology
of the infections and the second to the Management of the Biomedical Search.
The chosen teachers are two university professors of the University of
Wisconsin in Immunology and Pathology respectively. Their experience of
life and expertises investigators is different: one (David Pauza.) represents
the classic forty-years old American university professor, formed in the
best college and the university, with a strongly experience to use its
knowledge practically, not only in publishing but also in making patents,
technological transfer, acquiring Grant and manage the laboratory like
an industry.
The other, (Miroslav Malkovskhy) is an example of the European scientist
of the second half of the last century, as he has been educated first
in the faculty medicine in Prague at the time in which Czech immunology
was second only to the British immunology, then at Medical research Council
in London.. Finally, with the great migration of the Immunology from England
to the United States, Miroslav Malkovsky has been selected to teach Immunology
at the Department of Microbiology, Immunology and Pathology of the University
of Wisconsin.
V.
Forthcoming activities
For the future activities
as UNESCO UNITWIN Program, the Rector of the University of Rome "Tor
Vergata" has invited Dr.Josefina Calvo Quintana (Instituto de Materiales
y Reactivo Grupo de Sensores y Biosensores, Universidad de la Habana Cuba)
to spend 12 months in the Laboratory of Biosensor, co-ordinated by Professor
G. Palleschi, Director of Department of Chemical Sciences and Technologies
at the University of Rome " Tor Vergata". A specific courses
on biosensors in biotechnology will be held for the student of biology
and chemistry.
VI.
Development Prospects
The UNESCO Chair in Interdisciplinary Biotechnology at the University
of Rome "Tor Vergata" aims to enhance the integration of the
different fields of Biotechnology. In the first two years of activities
of the Chair, the selected topics have been Biology and Medicine. In the
next years Chemistry, Economy and Law will be approached with the students
of Biology and Medicine in Rome.
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